Journal: Biology of Sex Differences

Article Title: Bile acid distributions, sex-specificity, and prognosis in colorectal cancer

doi: 10.1186/s13293-022-00473-9

Figure Lengend Snippet: Sex differences in bile acid levels by comparing stage II RCC and LCC from patients aged ≥ 55 years

Article Snippet: Glycodeoxycholic acid (GDCA) and glycolithocholic acid (GLCA) were purchased from Toronto Research Chemicals (TRC, Canada).

Techniques:

Journal: Biology of Sex Differences

Article Title: Bile acid distributions, sex-specificity, and prognosis in colorectal cancer

doi: 10.1186/s13293-022-00473-9

Figure Lengend Snippet: Multivariable Cox regression associating 17 bile acid levels or ratio of conjugated bile acids to unconjugated bile acids and 5-year overall survival for patients ≥ 55 years old ( n = 197) adjusting for age, sex, tumor anatomic location, stage, and chemotherapy use

Article Snippet: Glycodeoxycholic acid (GDCA) and glycolithocholic acid (GLCA) were purchased from Toronto Research Chemicals (TRC, Canada).

Techniques:

Journal: Biology of Sex Differences

Article Title: Bile acid distributions, sex-specificity, and prognosis in colorectal cancer

doi: 10.1186/s13293-022-00473-9

Figure Lengend Snippet: Multivariable Cox regression associating 17 bile acid abundances or ratios to 5-year recurrence-free survival (RFS) for patients ≥ 55 years old ( n = 197) adjusting for age, sex, tumor anatomic location, stage, and chemotherapy use

Article Snippet: Glycodeoxycholic acid (GDCA) and glycolithocholic acid (GLCA) were purchased from Toronto Research Chemicals (TRC, Canada).

Techniques:

Journal: Biology of Sex Differences

Article Title: Bile acid distributions, sex-specificity, and prognosis in colorectal cancer

doi: 10.1186/s13293-022-00473-9

Figure Lengend Snippet: Pearson correlation of glucuronic acid to bile acid abundances in patients with right-sided colon cancer ≥ 55 years old ( n = 98)

Article Snippet: Glycodeoxycholic acid (GDCA) and glycolithocholic acid (GLCA) were purchased from Toronto Research Chemicals (TRC, Canada).

Techniques:

Journal: Scientific Reports

Article Title: Discovery of glycocholic acid and taurochenodeoxycholic acid as phenotypic biomarkers in cholangiocarcinoma

doi: 10.1038/s41598-018-29445-z

Figure Lengend Snippet: Comparison table of the average composition ratio (%) of 15 biliary bile acids in CCA, BBD and PC patients.

Article Snippet: Glycolithocholic acid (GLCA), tauroursodeoxycholic acid (TUDCA) and taurochenodeoxycholic acid (TCDCA) were obtained from Carbosynth (Berkshire, UK) and glycochenodeoxycholic acid (GCDCA), glycodeoxycholic acid (GDCA) and taurolithocholic acid (TLCA) were purchased from Santa Cruz (TX, USA).

Techniques: Comparison

Journal: Nutrients

Article Title: Effect of Standardized Grape Powder Consumption on the Gut Microbiome of Healthy Subjects: A Pilot Study

doi: 10.3390/nu13113965

Figure Lengend Snippet: Serum bile acid concentrations.

Article Snippet: Cholic acid (CA), chenodeoxycholic acid (CDCA), deoxycholic acid (DCA), lithocholic acid (LCA), ursodeoxycholic acid (UDCA), hyodeoxycholic acid (HDCA), taurocholic acid (TCA), taurochenodeoxycholic acid (TCDCA), taurodeoxycholic acid (TDCA), taurolithocholic acid (TLCA), glycocholic acid (GCA), glycochenodeoxycholic acid (GCDCA), glycodeoxycholic acid (GDCA), taurolithocholic acid (TLCA), glycodeoxycholic acid-d4 (GDCA-D4) and chenodeoxycholic acid-d4 (CDCA-D4) were purchased from Cayman Chemical (MI, USA).

Techniques:

Journal: Microorganisms

Article Title: Evaluation of the Probiotic Properties and the Capacity to Form Biofilms of Various Lactobacillus Strains

doi: 10.3390/microorganisms8071053

Figure Lengend Snippet: Bile salt hydrolase (BSH) activities. The six strains were tested for their ( a ) glycodeoxycholic acid (GDCA) and ( b ) taurodeoxycholic acid (TDCA) hydrolase activities. BSH activity was detected by the observation of an iridescent halo demonstrating the precipitation of deoxycholic acid. The presence or absence of the activity and growth capacity for each strain are summarized in .

Article Snippet: The six Lactobacillus strains were tested for taurodeoxycholic acid (TDCA) and glycodeoxycholic acid (GDCA) hydrolase activities on MRS agar plates supplemented with either 0.5% TDCA (Cayman Chemical Company, Ann Arbor, MI, USA) or 0.5% GDCA (Merck Millipore, Burlington, CO, USA), following the protocol described by Moser and Savage [ ].

Techniques: Activity Assay

Journal: Microorganisms

Article Title: Evaluation of the Probiotic Properties and the Capacity to Form Biofilms of Various Lactobacillus Strains

doi: 10.3390/microorganisms8071053

Figure Lengend Snippet: Bile salts activities and tolerance. The absence or presence of bile salt hydrolase (BSH) activity and tolerance to 0.5% taurodeoxycholic acid (TDCA) or glycodeoxycholic acid (GDCA) on agar plates were evaluated.

Article Snippet: The six Lactobacillus strains were tested for taurodeoxycholic acid (TDCA) and glycodeoxycholic acid (GDCA) hydrolase activities on MRS agar plates supplemented with either 0.5% TDCA (Cayman Chemical Company, Ann Arbor, MI, USA) or 0.5% GDCA (Merck Millipore, Burlington, CO, USA), following the protocol described by Moser and Savage [ ].

Techniques: Activity Assay

Journal: Frontiers in Microbiology

Article Title: Bile-Salt-Hydrolases from the Probiotic Strain Lactobacillus johnsonii La1 Mediate Anti-giardial Activity in Vitro and in Vivo

doi: 10.3389/fmicb.2017.02707

Figure Lengend Snippet: Substrate specificities of L. johnsonii La1 BSHs. (A) Hydrolase activity of rBSH47 produced in E. coli tested on LB plates. E. coli SE1 wild type or E. coli SE1 expressing rBSH47 were plated in the presence of taurodeoxycholic acid 0.3% (TDCA) or glycodeoxycholic acid 0.3% (GDCA) and incubated for 48–72 h. Activity is detected when a whitish halo forms around colonies. Data with E. coli SE1 expressing rBSH12 and rBSH56, respectively, are not shown. (B) Phylogenetic relationship among selected BSH sequences and substrate prediction. Programme MEGA5 was used for the phylogenetic tree and for the 500-replication bootstrap analysis. The following predicted amino acid sequences were obtained from UniProtKB/Swiss-Prot databases: BSH12, L. johnsonii La1 (Q74IV4_LACJO); BSH47, L. johnsonii La1 (Q74JG0_LACJO); BSH12, L. johnsonii La1 (Q74LX7_LACJO); CBAH-1, C. perfringens strain 13 (P54965.3); BSHA, L. acidophilus LA4 (ACL98173.1); BSHB, L. acidophilus LA4 (ACL98173.1); BSHA, L. acidophilus LA11 (ACL98175.1); BSHB, L. acidophilus LA11 (ACL98176.1); BSHA, L. acidophilus NCFM (YP_193782.1); BSHB, L. acidophilus NCFM (AAV42923.1); BSH , L. gasseri AM1 (ACL98172.1); BSH-α, L. johnsonii 100-100 (AAG22541.1); BSH-β, L. johnsonii 100-100 (AAC34381.1); BSHA, L. johnsonii pf01 (EGP12224.1); BSHB, L. johnsonii pf01 (EGP13287.1); BSHC, L. johnsonii pf01 (EGP12391.1); pCBH1, L. plantarum 80 (AAB24746.1); BSH1, L. plantarum WCSF (CCC80500.1); BlBSH, B. longum subsp. longum (2HF0); BSH, L. acidophilus PF01 (ABQ01980.1); BSH, L. plantarum CK 102 (Ha et al., ); Penicillin V Acylase (PVA), Bacillus sphaericus (3PVA). Substrate specificity, when known (see text for referenced literature) is indicated for each BSH enzyme (in brackets): (TC), specificity for tauro-conjugated bile salts; (GC), specificity for glyco-conjugated bile salts; (TC/GC), specificity for both tauro and glyco-conjugated bile salts.

Article Snippet: Overnight cultures were then spotted on LB agar plates supplemented with either 0.5% taurodeoxycholic acid (TDCA, Sigma-Aldrich) or 0.5% glycodeoxycholic acid (GDCA, Merck Millipore) and incubated at 37°C for 48 h. The BSH hydrolyzing activities were also monitored using purified recombinant enzymes in presence of conjugated bile salts, in solution, by measuring the liberation of amino acids (glycine or taurine) as previously described (Grill et al., ).

Techniques: Activity Assay, Produced, Expressing, Incubation